CY3熒光染料用哪個波段激發？

經常有同學咨詢，CY3熒光染料用哪個波長激發，發出來的是什么顏色的光，現將廠家的說明提供如下：

Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.

The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore. The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.

從上面資料可以看到，CY3熒光染料用532nm波段激發（綠光），發出來的是橙色光。

CY3的激發波長和發射波長

Photos of Streptavidin Cy3

Cy3染料是一種明亮的橙色熒光染料，可以使用532nm的激光譜線，并通過TRITC(四甲基羅丹明)濾光片來顯示。除了免疫細胞化學應用外，Cy3染料通常被用來標記核酸。Cy3屬于氨基反應染料。分子式為：暫無數據。分子量為：668.86